ER or Golgi?: Localization of ER Alpha 1,2-Mannosidase I
نویسندگان
چکیده
منابع مشابه
ER to Golgi Transport
The membrane transport factor p115 functions in the secretory pathway of mammalian cells. Using biochemical and morphological approaches, we show that p115 participates in the assembly and maintenance of normal Golgi structure and is required for ER to Golgi traffic at a pre-Golgi stage. Injection of antibodies against p115 into intact WIF-B cells caused Golgi disruption and inhibited Golgi com...
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To examine the role of early carbohydrate recognition/trimming reactions in targeting endoplasmic reticulum (ER)-retained, misfolded glycoproteins for ER-associated degradation (ERAD), we have stably expressed the cog thyroglobulin (Tg) mutant cDNA in Chinese hamster ovary cells. We found that inhibitors of ER mannosidase I (but not other glycosidases) acutely suppressed Cog Tg degradation and ...
متن کاملER-golgi traffic is a prerequisite for efficient ER degradation.
Protein quality control is an essential function of the endoplasmic reticulum. Misfolded proteins unable to acquire their native conformation are retained in the endoplasmic reticulum, retro-translocated back into the cytosol, and degraded via the ubiquitin-proteasome system. We show that efficient degradation of soluble malfolded proteins in yeast requires a fully competent early secretory pat...
متن کاملThe isolated ER-Golgi intermediate compartment exhibits properties that are different from ER and cis-Golgi
A procedure has been established in Vero cells for the isolation of an intermediate compartment involved in protein transport from the ER to the Golgi apparatus. The two-step subcellular fractionation procedure consists of Percoll followed by Metrizamide gradient centrifugation. Using the previously characterized p53 as a marker protein, the average enrichment factor of the intermediate compart...
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Chen, Jin Q., Michael Delannoy, Carol Cooke, and James D. Yager. Mitochondrial localization of ER and ER in human MCF7 cells. Am J Physiol Endocrinol Metab 286: E1011–E1022, 2004. First published January 21, 2004; 10.1152/ajpendo.00508.2003.—We observed previously that estrogen treatment increased the transcript levels of several mitochondrial DNA (mtDNA)-encoded genes for mitochondrial respira...
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ژورنال
عنوان ژورنال: Trends in Glycoscience and Glycotechnology
سال: 2014
ISSN: 0915-7352,1883-2113
DOI: 10.4052/tigg.26.103